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The intracellular metabolite extracts and 2 mL of spent culture medium containing extracellular metabolites were freeze-dried (BenchTop K manifold freeze dryer, VirTis) prior chemical derivatisation

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Hamilton LE, Suzuki J, Aguila L et al (2019) Sperm-borne glutathione-S-transferase omega 2 accelerates the nuclear decondensation of spermatozoa during fertilization in mice

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Samples were reduced with DTT and alkylated with iodoacetamide as described above, then diluted with DPBS (300 L), and taken forward to LysC and trypsin digestion without precipitation